[Molecular Pharmaceutics] Mitigation Strategies against Antibody Aggregation Induced by Oleic Acid in Liquid Formulations

We analyzed the destabilizing interactions between antibodies and oleic acid, resulting from the degradation of polysorbate 80 in therapeutic formulations. Antibodies rapidly adsorb to the oleic acid–water interface, forming a viscoelastic layer that triggers particle formation upon rupture, with the propensity being protein-specific. Our findings further reveal that PS80 prevents adsorption only under certain conditions, and adding l-arginine effectively mitigates interfacial particle formation, highlighting the need for tailored strategies to mitigate aggregation in antibody formulations. Have a look external page here!