[Analytical Chemistry] One-Step Immunoassay for Biomarker Quantification in Complex Mixtures Based on Phase-Separated Antifouling Coacervates

We develop and present a one-pot sandwich immunoassay (termedoneSTEP) to detect target biomolecules in complex biological fluids based onprogrammable zwitterionic polymer coacervates. We design these coacervates to selectively recruit target analytes with ultralow nonspecific adsorption. We show that dynamic compartmentalization combined with local target enrichment delivers a rapid and wash-free sandwich immunoassay with high specificity and a high signal-to-noise ratio. Read more external page here!