Scalable production and isolation of extracellular vesicles

All cells send biological information to other cells by releasing tiny membrane-bound vesicles called extracellular vesicles, which are extremely attractive for many applications including therapeutics and diagnostics. Currently, interest in the field is growing exponentially, but the low vesicle yields and lack of suitable characterization technologies are slowing down and complicating research activities.
Our aim is to develop novel technologies for the production, characterization and engineering of extracellular vesicles, eventually leading to the manufacturing of reproducible extracellular vesicles on a larger scale. Our project is part of the European consortium BOW, funded by the Fet-Open call of Horizon2020 Programme and coordinate by Dr. Paolo Bergese (Università di Brescia, Italy).

Contacts

Carolina, Karl

Key publications

external pageC.Paganini et al. "High‐Yield Separation of Extracellular Vesicles Using Programmable Zwitterionic Coacervates." Small, 2022call_made

external pageC. Paganini et al. "High‐yield Production of Extracellular Vesicle Subpopulations with Constant Quality Using Batch‐refeed Cultures." Advanced Healthcare Materials, 2022call_made

external pageC. Paganini et al. "Rapid Characterization and Quantification of Extracellular Vesicles by Fluorescence-Based Microfluidic Diffusion Sizing." Advanced Healthcare Materials, 2021call_made